In Vitro Effects of Antioxidant and Proapoptotic Activities of Thymoquinone Iron Complex

Abstract

This study aimed to investigate the proapoptotic and antioxidant effects of the Thymoquinone (TQ) iron complex on the SW480 cell line. This study investigates the proapoptotic and anti-oxidant effects of the TQ iron complex on the SW480 cell line. The SW480 cells were routinely cultured in a medium for 48 h. and incubated at 37°C in a 5% CO2 in the air. After the incubation period, the cells were washed with buffer, and 100 ml of the denaturing lysis buffer per 0.5 was added to 2x107 cells for 15 min, and supernatants were taken. ELISA test was used to examine the expression and activity of GADD153, Wee1, cleaved Caspase-3, Bax, GRP78, and Bcl-2 proteins in SW480 cells. In this study, to measure activities of total antioxidant capacity (TAS), catalase (CAT), total oxidant capacity (TOS), and superoxide dismutase (SOD) activities were investigated by the ELISA method in cell lines SW480 treated with the TQ iron complex. ELISA test results indicated that the activities of apoptotic proteins Bax, Wee1 Caspase-3, GADD153, GRP78, and Bcl-2 in human SW480 cell lines were significantly increased in the 48-hour treatment. Our results of this study demonstrated that in untreated cultures, high TAS, SOD and CAT activities were found in SW480 cell lines than in control cell lines.