Abstract
The present study aimed to investigate the beneficial of prepared black rice anthocyanins nano-composite (An-AgNps) against hepatotoxicity induced by methotrexate (MTX) in rats. Anthocyanins nano-composite was prepared by silver as the metallic ion reduction and were characterized by IR and SEM. The rats in our experiment were divided into five groups. Serum lipid profile, serum transaminases (ALT and AST), ALP, LDH, TBA, GSH and SOD were examined. The results show that SEM of An-AgNps has average particle size from 70 to 130nm. In the group treated with MTX; TC, TG, LDL-c, ALT, AST, ALP, LDH and TBA levels were significantly (P≤0.05) increased than NC, while, HDL-c, SOD and GSH levels were significantly (P≤0.05) decreased. On the other hand, An-AgNps + MTX treated groups were reversed the levels of all biomarkers similar to NC. In conclusion, the results show that An-AgNps has a protective effect on MTX-induced hepatotoxicity and oxidative stress.
Highlights
Methotrexate (MTX) (4-amino-N10-methyl folic acid) has been used in the various therapy types of diseases includingpsoriasis, cancers immunologic disorders, Crohn’s disease, rheumatoid arthritis, for the multiple sclerosis treatment, sarcoidosis, dermatomyositisand stage of various cancerous (Ozogul et al, 2013; Vardi et al, 2010; Celik et al, 2013)
G1; Normal Control (NC) group, G2; Positive Control (PC) group was treated with single dose of MTX (20 mg/kg.b.w) i.p injection, G3; treated group was treated with single dose of MTX (20 mg/kg.b.w) i.p injection + An-AgNPs (10 mg/kg.b.w), G4; treated group was treated with single dose of MTX (20 mg/kg.b.w) i.p injection + An-AgNPs (15 mg/kg.b.w), G5; treated group was treated with single dose of MTX (20 mg/kg.b.w) i.p injection + An-AgNPs (20 mg/kg.b.w)
The value of thiobarbituric acid (TBA) was observed by measuring the difference between absorbance value at 520 and at 532 nm, which represents the concentration of malondialdehyde, and was taken as the measure of lipid peroxidation
Summary
Methotrexate (MTX) (4-amino-N10-methyl folic acid) has been used in the various therapy types of diseases includingpsoriasis, cancers immunologic disorders, Crohn’s disease, rheumatoid arthritis, for the multiple sclerosis treatment, sarcoidosis, dermatomyositisand stage of various cancerous (breast cancer, lung cancer, head and neck tumours, lymphoma, leukemia, osteosarcoma, etc.) (Ozogul et al, 2013; Vardi et al, 2010; Celik et al, 2013). In all the metals the pure silver has the highest thermal conductivity, and electrical, and has Many studies reported that the extracts of anthocyanin from plants and natural food have hepatoprotective effect and believed to be beneficial to liver health, because they have strong antioxidant activity (Sankhari et al, 2012). Our present study aimed to increase the stability of anthocyanins by synthesis anthocyanins silver nanoparticles, characterize the prepared nanoparticles and investigate its protective effects on liver hepatotoxicity induced by Methotrexate
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