Abstract
BackgroundThe association of rs17321515 single nucleotide polymorphism (SNP) near TRIB1 gene and serum lipid profiles has never been studied in the Chinese population. Therefore, the present study was undertaken to detect the association of rs17321515 SNP and several environmental factors on serum lipid levels in the Mulao and Han populations.MethodsA total of 639 unrelated subjects of Mulao nationality and 644 participants of Han nationality were randomly selected from our previous stratified randomized cluster samples. Genotypes of the TRIB1 rs17321515 A>G SNP were determined via polymerase chain reaction and restriction fragment length polymorphism, and then confirmed by direct sequencing.ResultsSerum apolipoprotein (Apo) B levels were higher in Mulao than in Han (P < 0.05). There were no differences in the genotypic and allelic frequencies between the two ethnic groups (P > 0.05). High- and low-density lipoprotein cholesterol (HDL-C and LDL-C) levels in Han were different among the genotypes (P < 0.05 for each), the subjects with AG/GG genotypes had higher HDL-C and LDL-C levels than the subjects with AA genotype. Total cholesterol (TC), HDL-C, LDL-C, ApoA1 and ApoB levels in Han males were different among the genotypes (P < 0.05-0.001), the G carriers had higher TC, HDL-C, LDL-C, ApoA1 and ApoB levels than the G noncarriers. HDL-C levels in Mulao males were different among the genotypes (P < 0.05), the G carriers had lower HDL-C levels than the G noncarriers. Serum HDL-C and LDL-C levels in both ethnic groups and TG levels in Han were correlated with the genotypes or alleles (P < 0.05-0.01). TG and HDL-C levels in Mulao males and TG, HDL-C, LDL-C and ApoA1 levels in Han males were correlated with genotypes or alleles (P < 0.05-0.001). TG and ApoA1 levels in Han females were associated with genotypes (P < 0.05 for each). Serum lipid parameters were also associated with several environmental factors in both ethnic groups.ConclusionsThe associations of TRIB1 rs17321515 SNP and serum lipid levels are different between the Mulao and Han populations. These discrepancies might partly result from different TRIB1 gene-environmental interactions in both ethnic groups.
Highlights
Over decades, it has evident that the most attributable and heritable risk factor for coronary artery disease (CAD) is the unfavorable serum lipid profile such as elevated serum levels of total cholesterol (TC) [1], triglyceride (TG) [2], variation in plasma lipid phenotypes can be explained by genetic polymorphisms [12,13,14].It is noticeable that through genome-wide association studies, a growing number of new loci involving in lipid metabolism have been identified [15,16,17]
The associations of TRIB1 rs17321515 single nucleotide polymorphism (SNP) and serum lipid levels are different between the Mulao and Han populations
One study demonstrating the role of TRIB1 in lipoprotein metabolism in mice reported that TRIB1 overexpression significantly reduced the plasma levels of very low-density lipoprotein (VLDL), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C) and TG; TRIB1 deficiency experienced the reverse effects on plasma cholesterol and TG in vise versa [23]
Summary
It has evident that the most attributable and heritable risk factor for coronary artery disease (CAD) is the unfavorable serum lipid profile such as elevated serum levels of total cholesterol (TC) [1], triglyceride (TG) [2], variation in plasma lipid phenotypes can be explained by genetic polymorphisms [12,13,14].It is noticeable that through genome-wide association studies, a growing number of new loci involving in lipid metabolism have been identified [15,16,17]. The minor allele at this SNP was found to be associated with an atheroprotective lipid phenotype of lower TG and LDL-C, and higher HDL-C resulting in a significantly reduced risk of CAD in European [7], Malays and Asian Indians [24]. This association was genome-wide significant in severe hypertriglyceridemic subjects [25]. The association of rs17321515 single nucleotide polymorphism (SNP) near TRIB1 gene and serum lipid profiles has never been studied in the Chinese population. The present study was undertaken to detect the association of rs17321515 SNP and several environmental factors on serum lipid levels in the Mulao and Han populations
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