Synthesis, Characterization and Pharmacological Evaluation of Ethanolic Extract of Sarcostemma brevistigma for its Anti-inflammatory and Antipsoriatic Activity

Abstract

The goal of this work was to test the anti-inflammatory and anti-psoriatic properties of an ethanolic extract of Sarcostemma brevistigma stem in the formation of a hydrogel utilizing several mouse models. The methanolic fraction was obtained by fractionating an ethanolic extract of Sarcostemma brevistigma stem with methanol. Viscosity, spreadability, drug release profile, pH, homogeneity, physical properties and irritant impact of the prepared hydrogel were assessed. Percentage inhibition, nitric oxide (NO), and myeloperoxidase (MPO) levels in mice ears following acute inflammation caused by croton oil were all measured. Physical examination revealed that the produced hydrogels were brownish in color and had a homogenous and smooth look when applied. All other test criteria, including as pH, viscosity, spreadability, and consistency, were determined to be acceptable in the F3 hydrogel formulation. The optimal F3 formulation component was 1.5 g of Carbopol 940 and 1% sodium CMC. In xylene-induced ear edema in mice, the percent suppression of edema was found to be equivalent to the standard group of therapy (69.58 %). The results of this investigation show that a produced hydrogel of SB extract has inhibitory activity on acute inflammation. The capacity of croton oil to promote neutrophil influx in mouse ear tissue was shown in this study. Treatment with extract hydrogels, as well as Voltaren Emulgel, substantially decreased MPO and NO levels in mice ears (P < 0.01). The presence of flavonoids in Sarcostemma brevistigma may explain why the methanol extracts have a substantial anti-inflammatory effect.