Formulation and Evaluation of Ocimum Sanctum based Emulgel for Wound Healing Potential

Abstract

The purpose of this study was to evaluate the anti-inflammatory effect of leaves of Occimum sanctumin the formulation of hydrogels using different types of animal models. Materials and Methods: O. sanctum leaf ethanol extract was fractionated with methanol to give a methanol fraction. The methanol fraction (1% vw / w) was used in hydrogel formulations in various combinations of Carbopol 940 and sodium CMC. The hydrogels produced were characterized for optimal physical properties, pH, ductility, uniformity, viscosity, release profile, and irritation. Optimized hydrogels were evaluated for anti-inflammatory activity in animals using xylene-induced, croton oil-induced ear edema, and cotton pellet-induced granuloma models. The effect was monitored by , which measures the suppression rate of ears , nitric oxide (NO) and myeloperoxidase (MPO) levels in mice after acute inflammation induced by croton oil. Results and discussion: Physical evaluation confirmed that the resulting hydrogel was brownish in color and had a uniform and smooth appearance when applied. The combination of F3-hydrogel formulations was found to be appropriate for all other evaluation parameters such as pH, viscosity, spreadability and consistency. Therefore, it was observed that the optimal composition of F3 preparation is 1.5 g of Carbopol 940 and 1% sodium CMC. The suppression rate of edema in xylene-induced ear edema in mice was found to be comparable to that of the standard treatment group (65.59%). In this study, the results show that the prepared hydrogel-OSMFH has an inhibitory effect on acute inflammation. The results show the ability of croton oil to induce the influx of neutrophils into the ear tissue of mice.