Synthesis, characterization and evaluation of swelling ratio on magnetic p53-poly(MAA-co-EGDMA)@GO-Fe3O4 (MIP@GO-Fe3O4)-based p53 protein and graphene oxide from kusambi wood (Schleichera oleosa)

Abstract

The p53 protein plays an important role by acting as a transcription factor to regulate gene expression and its genetic mutation has been reported as the most common genetic alterations in human cancer diseases. This opens possibilities for the development of early identification of cancer by utilizing p53 protein as tumor biomarkers. In this work we report the synthesis, characterization and evaluation of p53-poly(MAA-co-EGDMA)@GO-Fe3O4 as SPE materials for selective extraction of wild p53 protein. The magnetic material p53-poly(MAA-co-EGDMA)@GO-Fe3O4 (MIP@GO-Fe3O4) is successfully prepared with GO-Fe3O4 from kusambi wood (Schleichera oleosa) as the host material, p53 as a template molecule, methacrylic acid (MAA) as a monomer, ethylene glycol dimethacrylate (EGDMA) as a crosslinker, benzoyl peroxide (BPO) as an initiator and acetone/ethanol as a porogen. Synthesis is carried out using a bulk polymerization method. To find out the best ratio that produces the highest MIP@GO-Fe3O4 mass, the five variations of the volume ratio of template, monomer and crosslinker (P1–P5) are investigated. It was found that the volume ratio of template, monomer and crosslinker that produced the highest MIP@GO-Fe3O4 mass is obtained at P3 volume ratio (1:4:8mL, which is further polymerized with GO-Fe3O4 as much as 0.6239g). Swelling ratio studies using 3 (three) solvents namely chloroform, acetone and methanol show that the swelling ratio values obtained are 1.46, 1.24 and 1.23g/g, respectively. These results indicate that methanol is the best solvent for use in the rebinding process of MIP@GO-Fe3O4 material. Based on the analysis by FTIR, XRD, SEM-EDX and BET-BJH, the resulting MIP@GO-Fe3O4 material is expected to be used later for wild p53 preconcentration from various biological samples.