Abstract
In this research, molecular imprinting polymers (MIPs) for D-arabinitol were synthesized using a bulk polymerization method through a noncovalent approach. The MIPs were prepared by using D-arabinitol as a template, acrylamide as a functional monomer, ethylene glycol dimethacrylateas cross-linker, benzoyl peroxide as an initiator and dimethyl sulfoxideas a porogen. MIPS was synthesized in several formulas with a different molar ratio of template to functional monomers and cross-linker. Fourier-transform infrared spectroscopy (FT-IR) and scanning electron microscopy (SEM) were used to characterize the MIPs produced. A batch rebinding assay was used to test the binding efficiency of each formula. Batch rebinding test results revealed that MIPsF3 with a molar ratio of the template: monomer and crosslinker ratio respectively (1: 4: 25) had the highest binding capacity at 1.56 mgg -1 . The results of isotherm adsorption showed that the MIPs produced followed the Freundlich equation with an R-value of 0.97. The MIPs produced was also selective toward its isomeric compounds (i.e. L-arabinitol, adonitol, xylitol, and glucose). The extraction efficiency of the MIPs against D-arabinitol was 88.98%.
Highlights
D-arabinitol is a typical metabolite product of several Candida species that are pathogenic
The reaction of polymer formation between functional monomers and template occurred in situ in noncovalent interactions
The initiator used in this molecular imprinting polymers (MIPs) synthesis was a benzoyl chloride solution in chloroform with DMSO acting as a porogen
Summary
D-arabinitol is a typical metabolite product of several Candida species that are pathogenic. The levels of Darabinitol in serum and urine increase if the fungus Candida proliferates in the organism and causes invasive candidiasis [1]. In the late 1970s, high levels of D-arabinitol were found in the blood of systemic candidiasis patients. This fact made D-arabinitol potentially useful as a diagnostic tool in candidiasis patients. The incorporation of D-arabinitol as a diagnostic tool achieved a low specificity because the increased levels of D-arabinitol in the blood were found in candidiasis patients and patients suffering from kidney dysfunction. To avoid false-positive results due to kidney damage, D-arabinitol levels are usually expressed in terms of the ratio D-arabinitol to creatinine or the ratio of D- to L-arabinitol [2,3,4,5,6,7,8,9]
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