Abstract
The interaction between methylene blue–Dy(III) complex and herring sperm DNA was investigated in physiological buffer (pH 7.40). The binding ratios and the apparent molar absorptivity of ϵ MB-Dy-DNA were determined by the molar ratio method. The thermodynamic parameters were calculated by a double-reciprocal equation at different temperatures, and showed that the interaction of methylene blue–Dy(III) complex with DNA was driven mainly by Δr S Θ m. By combination analysis of the Scatchard method, viscosity measurements, ferrocyanide quenching experiments, and circular dichroism spectroscopy, the methylene blue–Dy(III) complex can bind to DNA, and the major binding mode is electrostatic and partial intercalation binding.
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