Abstract
Background: Dental Composites despite being the leading aesthetic direct restorative materials have a short life expectancy due to failure at tooth restoration interface. The restorative materials and techniques used in dentistry are not able to immediately remineralize nor protect demineralized dentin nor improve on the poorly resin-infiltrated hybrid layer.Objective: To characterize and assess the cellular viability of proprietary bioactive glass (BAG) modified with zinc oxide and boron oxide BAG, prepared through molten salt ion exchange method.Materials and Methods: Commercial available 45S5 bioactive glass (XL Sci-Tech.USA) was modified by adding zinc acetate and boric acid separately in 10 and 20 wt %. The powder was annelid at 400 °C for 1 h in a glass furnace. After drying the powder was sifted through 100 µm fine sieve. The XRD, FTIR, EDX and SEM of both commercial and experimentally modified BAG were carried out to characterize the powder before and after storage in SBF for 7 days. Cell vitality was assessed using MTT assay at three different concentrations (10µg/ml, 50µg/ml and 100µg/ml) in alpha MEM media from a stock solution of 10mg/ml.Results: SEM, EDX, XRD and FTIR confirmed hydroxyapatite after 7 day soaking in SBF. In vitro cell vitality was confirmed through MTT easy. Maximal cell vitality was shown at in 2ZA group (106.5%). While comparing the MTT easy results, multiple pair t-test were found to be statistically non-significant (P < .05).Conclusion: The present study molten salt ion exchange method successfully modified BAG. The modified BAG displayed enhanced bioactivity after 7 day soaking in SBF. MTT easy showed improve in-vitro cell vitality, with highest cell proliferation such that 2ZA >1BAwith1ZA>1BA. Keywords: Bioactive glass, Bioactivity, Boron Oxide, MTT easy, Surface modifications, Zinc Oxide
Published Version
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