SYNTHESIS AND STUDY OF THE OPTICAL PROPERTIES OF THE COLLOIDAL FORM OF IRON WITH RIBOFLAVIN AND L-LYSINE

Abstract

This paper presents the results of the development and study of a colloidal complex of the essential microelement iron with vitamin B2 riboflavin and the essential amino acid L-lysine. As a result, a method for obtaining a colloidal vitamin-microelement-amino acid complex has been developed, which is carried out in 2 stages. As a result of computer quantum chemical modeling, the most optimal configuration for the interaction of the components of the colloidal complex was determined. Namely, it was found that the connection of riboflavin with the iron ion occurs through oxygen at C4 and nitrogen N3, and lysine binds to the iron ion through the carboxo and amino group in α- position. The optical characteristics of the resulting compound were studied. From an analysis of the absorption spectrum of a 0.001% aqueous solution of an iron complex with riboflavin and L-lysine, the presence of 4 maxima in the spectrum with positions at 222, 265, 374 and 436 nm was established. In the spectrum, the absorption maximum at a wavelength of 436 nm corresponds to the auxochromic amino group; its hypsochromic shift by 20 nm relative to the absorption spectrum of pure riboflavin confirms the formed bond of the amino group with the iron ion in the complex molecule. The fluorescence maximum is located at a wavelength of 522 nm, which corresponds to intense green-yellow fluorescence in both a solution of pure riboflavin and a colloidal iron complex with riboflavin and L-lysine. Analysis of the histogram of the size distribution of micelles of the complex showed the presence of one phase with a size of about 96 nm, which allows us to define the resulting iron complex with riboflavin and the amino acid L-lysine as a colloidal system. For citation: Blinov A.V., Golik A.B., Rekhman Z.A., Gvozdenko A.A., Kolodkin M.A., Bakholdina T.N. Synthesis and study of the optical properties of the colloidal form of iron with riboflavin and L-lysine. ChemChemTech [Izv. Vyssh. Uchebn. Zaved. Khim. Khim. Tekhnol.]. 2024. V. 67. N 7. P. 96-102. DOI: 10.6060/ivkkt.20246707.6999.