Abstract
Substituted aryl acridinium esters, 2,6-dimethyl-4-(2-succinimidyloxycarbonylethyl)phenyl 10-methylacridinium-9-carboxylate trifluoromethanesulphonate ( 3), 2,6-dimethoxy-4-(2-succinimidyloxycarbonylethyl)phenyl 10-methylacridinium-9-carboxylate trifluoromethanesulphonate ( 4), 2-methoxy-4-(2-succinimidyloxycarbonylethyl)phenyl 10-methylacridinium-9-carboxylate trifluoromethanesulphonate ( 5) and 2,6-dibromo-4-(2-succinimidyloxycarbonylethyl)phenyl 10-methylacridinium-9-carboxylate trifluoromethanesulphonate ( 6), have been synthesised and coupled to immunoglobulin G (IgG). The chemiluminescent properties and hydrolytic stabilities of the conjugates have also been examined. The different substituents on the phenoxy ring affected the chemiluminescent efficiency and kinetics. All the human IgG conjugates of these substituted acridinium esters demonstrated much higher stability than the conjugate of the corresponding unsubstituted acridinium ester.
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call