Abstract
A novel chiral stationary phase hase been synthesized by grafting L-valinetert.-butylamide on to modified polycyanopropylmethyl phenylmethyl silicone (OV-225). This chiral phase can be used for the separation of amino acid enantiomers at temperatures ranging from 60° to 230°. This allows the separation of the protein amino acid enantiomers in one run. The elution properties of six derivatives (O-isopropyl, O- n-butyl, N-trifluoroacetyl, N-pentafluoropropionyl, N-heptafluorobutyryl) on the new chiral phase are compared.
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