Abstract
Silver nanostructures were successfully synthesized through a simple and “green” method using saccharides as reducing and capping agent. Transmission electron microscopy (TEM) and UV–Vis absorption were used to certify the quality of the silver nanoparticles obtained: firstly, size and dispersion. In this work Silver NanoParticles (AgNPs) cytotoxicity related to saccharides capping (Glucose and Glucose-Sucrose) was explored in human epitheloid cervix carcinoma cells (HeLa). The cells were incubated with increasing AgNPs number/cell and HeLa cells viability was monitored for a period of 48 h compared with the positive and negative controls. We observed that the toxicity increases with incubation time and with AgNPs number/cell. In particular, the different cytotoxic degree of the AgNPs, i.e. AgNP-G are more toxic than AgNP-GS, suggests that the cytotoxic effects are largely depended on the capping agent. The highest concentration of AgNP-G number/cell is able to induce extensive cell death of HeLa cells soon after 1 hr of incubation; conversely the lowest concentration of Ag NP-GS number/cell, surprisingly, is able to induce cell proliferation.
Highlights
Various types of nanomaterials exhibit new electrical, catalytic, magnetic, mechanical, photonic, and thermal properties to designnew device useful for a variety of scientific and technological sectors
The optical properties of metallic nanoparticles ranging from microclusters to nanoparticles have been investigated mainly on the size effects concerning the shift of the surface plasmon (SP) resonance and the variation of the SP bandwidth
The cytotoxicity of AgNPs in aqueous β‐D glucose (Fig. 4) and β‐D glucose‐sucrose (Fig. 4) solution was in vitro evaluated in human epitheloid cervix carcinoma cells (HeLa) cells at different incubation times, AgNPs/cell number and saccharides concentrations and it was indirectly measured by the MTT assay. Both for AgNPs in aqueous β‐D glucose and β‐D glucose‐ sucrose solution, the analyses showed a direct dose‐ response relationship, i.e. cytotoxicity increases with the AgNPs/cell number and incubation time
Summary
Various types of nanomaterials exhibit new electrical, catalytic, magnetic, mechanical, photonic, and thermal properties to designnew device useful for a variety of scientific and technological sectors. The number of products containing nanomaterials and their possible applications continue to grow exponentially. Nanomaterials are already being used or tested in a wide range of consumer products such as sunscreens, composites, and medical and electronic devices, and they are being used as chemical catalysts. To nanotechnology’s success in consumer products and other sectors, nanomaterials have promising environmental impact applications [1,2,3]. Nanosized cerium oxide has been developed to decrease diesel emissions, and iron nanoparticles can remove contaminants from soil and groundwater. Most of the natural processes take place in the nanometer scale regime. A synergy of nanotechnology and biology could solve several biomedical problems and revolutionize the health and medicine fields[4]
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