Rapid Biosynthesis of Silver Nanoparticles Using Pepino (Solanum muricatum) Leaf Extract and Their Cytotoxicity on HeLa Cells.

Mónica Gorbe,Jaime Prohens,M Marcos,Elena Aznar,Ramón Martínez-Máñez,Félix Sancenón,Abbaraju Venkataraman,Ravishankar Bhat,F Herraiz

doi:10.3390/ma9050325

Abstract

Within nanotechnology, gold and silver nanostructures have unique physical, chemical, and electronic properties [1,2], which make them suitable for a number of applications. Moreover, biosynthetic methods are considered to be a safer alternative to conventional physicochemical procedures for both the environmental and biomedical applications, due to their eco-friendly nature and the avoidance of toxic chemicals in the synthesis. For this reason, employing bio routes in the synthesis of functionalized silver nanoparticles (FAgNP) have gained importance recently in this field. In the present study, we report the rapid synthesis of FAgNP through the extract of pepino (Solanum muricatum) leaves and employing microwave oven irradiation. The core-shell globular morphology and characterization of the different shaped and sized FAgNP, with a core of 20–50 nm of diameter is established using the UV-Visible spectroscopy (UV-vis), field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM) and Zeta potential and dynamic light scanning (DLS) studies. Moreover, cytotoxic studies employing HeLa (human cervix carcinoma) cells were undertaken to understand FAgNP interactions with cells. HeLa cells showed significant dose dependent antiproliferative activity in the presence of FAgNP at relatively low concentrations. The calculated IC50 value was 37.5 µg/mL, similar to others obtained for FAgNPs against HeLa cells.

Highlights

Metal nanoparticles (MNP) present unique physical, chemical, electronic, and antibacterial properties which make them appropriate for their application in fields including biosensing [3], photonics [4], electronics [5], antimicrobials [6], and in the biomedical field for drug delivery and targeting [7].Materials 2016, 9, 325; doi:10.3390/ma9050325 www.mdpi.com/journal/materialsfunctionalization of nanoparticles through the conjugation of certain chemicals is known to allow specific recognition of certain molecules and biomolecules, which may enhance efficacy of nanoparticles in specific applications and reduce side health and environment effects
A reductant leaf extract was obtained from freshly collected leaves of pepino (S. muricatum) and exposed to microwaves using a conventional microwave oven at a frequency of 2.45 GHz for 180 s to denaturalize the enzymes and proteins present in the solution
In the present investigation we successfully developed an environmental friendly synthesis method for the production of biosynthesized FAgNP by exploiting S. muricatum leaf extract as a potential bioreductant

Summary

Introduction

Functionalization of nanoparticles through the conjugation of certain chemicals is known to allow specific recognition of certain molecules and biomolecules, which may enhance efficacy of nanoparticles in specific applications and reduce side health and environment effects. Developing a reliable experimental protocol for the synthesis of FMNPs is a challenging issue in current nanotechnology research, in the context of the recent drive to promote green technologies for their synthesis [8]. In this field, drawbacks associated with chemico-physical methods for the synthesis of. The ever growing need to develop a clean, nontoxic, and environmentally safe production processes for nanoparticles to reduce environmental impact, minimize waste, and increase energy efficiency has become essential in this field [9]

Methods

Results

Conclusion