Abstract
A new binuclear complex, [Zn2L2Cl4]·2H2O {L = N-aldehyde-N-(4-(benzyloxy)benzyl)-1,4,7triazacyclononane}, was synthesized and characterized by X-ray, elemental analysis, infrared and electronic spectroscopy, and mass spectrometry. The central ion is bridged by the L and lies in a tetrahedral configuration with Zn···Zn distance of 6.283 Å. The complex crystallizes in the triclinic space group Pī. ESI-MS of the complex indicates that the protonated ligand HL+ is the active species. The interaction of HL+ with calf thymus–DNA (CT–DNA) and bovine serum albumin (BSA) was studied by means of various spectroscopic methods, which revealed that HL+ could interact with CT–DNA through groove-binding mode and could quench the intrinsic fluorescence of BSA in a static quenching process. DNA–cleavage experiments indicate that HL+ exhibits efficient DNA–cleavage activity in the presence of H2O2, hydroxyl radical (HO•) may serve as the major cleavage active species, and the pseudo-Michaelis–Menten kinetic parameters (Kcat, KM, Vmax); 2.47 h−1, 2.70 × 10−4 M and 6.68 × 10−4 Mh−1.
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