Synthesis and characterizations of a highly sensitive and selective fluorescent probe for hydrogen sulfide

Abstract

Hydrogen sulfide (H2S) is an important endogenous signaling molecule with a variety of biological functions. To detect H2S in living biological systems, herein we developed a new fluorescent probe for highly sensitive and selective sensing of H2S in cells. The probe is based on coumarin–triazole as the fluorophore and thiolysis of the NBD (7-nitro-1,2,3-benzoxadiazole) amine as the receptor. Bioimaging experiments indicated that this probe could be used to monitor H2O2-induced H2S biosynthesis in yeast cells. Our results show that such thiolysis of the NBD amine can be used for the development of fluorescent H2S probes.