P107 Ratiometric fluorescent probe for selective detection of hydrogen sulfide in living cell

Abstract

Fluorescent probe for hydrogen sulfide should be a powerful tool to detect its functions in biological system. In this presentation, we introduce the development of the dual emission fluorescent probe for hydrogen sulfide based on a unique fluorescence sensing mechanism (i.e., AM-contact mechanism). Our fluorescent probe contained a cadmium ion, which is known to have a strong binding affinity for hydrogen sulfide. Treatment of the probe with hydrogen sulfide induced a clear and large ratiometric signal change within a few minute, which is due to the dissociation of the cadmium ion from the probe, as the result of the formation of the stable cadmium sulfide (CdS). Our probe selectively detected hydrogen sulfide among various biological relevant thiol species including glutathione and cysteine. This selectivity was successfully applied to detect endogenous hydrogen sulfide produced by CSE in living cells.