Synthesis and Characterization of Epigallocatechin Gallate-mediated Hydroxyapatite.

Abstract

Hydroxyapatite is a significant material that finds its application in the field of dental and bone tissue engineering. The formulation of nanohydroxyapatite with the aid of bioactive compounds has gained importance in recent years due to the beneficial activity contributed by them. The present work focuses on the formulation of nanohydroxyapatite synthesis using epigallocatechin gallate, an active biochemical component of green tea. The prepared epigallocatechin gallate-mediated nanohydroxyapatite (epi-HAp) was nanoglobular in shape and composed of calcium, phosphorous, carbon and oxygen, which was confirmed by Scanning electron microscope- energy dispersive X-ray analysis (SEM-EDX). The Attenuated Total Reflection-Infra red spectroscopy (ATR-IR) and X-ray photoelectron spectroscopy (XPS) assured that the reduction and stabilisation of nanohydroxyapatite were mediated by epigallocatechin gallate. The epi-HAp exhibited anti-inflammatory behaviour along with nil effect on cytotoxicity. To be precise, the epi-HAp can be an effective biomaterial in bone and dental applications.