Synthesis and Characterization of Copper Nanoparticles: Investigating Antibacterial Potential via Size and Surface Charge Modulations

Abstract

This research delves into a comprehensive investigation of copper nanoparticle synthesis and characterization, with a specific emphasis on exploring their antibacterial potential by manipulating size and surface charge. Employing a customized approach, precise control over nanoparticle dimensions and surface properties was achieved. Variations in size were attained by fine-tuning reaction parameters, while surface charge modifications were implemented through ligand functionalization. Thorough characterization using diverse analytical techniques, such as transmission electron microscopy (TEM), dynamic light scattering (DLS), zeta potential measurements, and Fourier-transform infrared spectroscopy (FT-IR), was conducted to elucidate morphological aspects and surface features. This multifaceted characterization aimed to provide a comprehensive understanding of the synthesized copper nanoparticles. Antibacterial assessments against various bacterial strains, spanning Gram-positive and Gram-negative species, were carried out, and the results were meticulously correlated with variations in nanoparticle size and surface charge. The outcomes not only advance the knowledge of copper nanoparticle synthesis but also shed light on the intricate relationship between nanoparticle properties and their antibacterial efficacy. This research holds promise for the development of tailored antibacterial agents with specific physicochemical properties, offering potential applications in antimicrobial materials and biomedical interventions, contributing to the evolving landscape of nanotechnology-driven solutions for combating bacterial infections.