Abstract
Syntheses, absorption and fluorescence properties for a series of new enzyme substrates are described, which are derived from 7-hydroxycoumarins possessing electron-withdrawing substituents in position 3. The new substrates are advantageous over existing ones in that they exhibit longwave absorption and fluorescence maxima as well as largeStokes' shifts. In addition, theirpK a values, which are usually between 6.0 and 7.0, allow the direct and continuous kinetic assay of hydrolases such as esterases, phosphatases, and sulfatases.
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