Abstract

MoO3-x NPs was rapidly synthesized at room temperature by an easy stirring method. It was interestingto find that MoO3-x NPs induce OH- to generate active free radicals (ROS), which isa highly promising property in chemiluminescence (CL). Benefiting from the abundant oxygen vacancy, MoO3-x NPs adsorbsH2O2 and turn it into ·OH. The oxidase activity of fluorescein under visible light had already been reported, which catalyzesdissolved oxygen to become O2-· and continue to convert to H2O2. By creating the synergy effect with fluorescein, MoO3-x NPs strengthen the CL intensity of K3[Fe(CN)6]-fluorescein system significantly. Utilizing the quench effect of uric acid for the CL intensity, we developed a rapid, simple, and highly sensitive CL platform for uric acid detection. The linear range was 5-80µM and the detection limit (LOD) for uric acid was 3.11µM (S/N = 3). This work expanded the application of MoO3-x NPs in the CL field and developed a simple and highly sensitive CL sensing system to detect UA in human saliva.

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