Synergistic effects of Woodfordia fruticosa silver nanoparticles accelerating wound healing in Swiss mice in vivo

Abstract

BackgroundSilver nanoparticles (AgNPs) have gained significant attention in recent years due to their unique physicochemical properties and potential applications in various fields, including medicine, catalysis, and environmental remediation. These nanoparticles possess antimicrobial, anti-inflammatory, and wound-healing properties, making them particularly promising for biomedical applications. The drug Woodfordia fruticosa (Wf) has been traditionally used in folk medicine for its therapeutic properties, such as wound healing, antimicrobial, anti-inflammatory, and antioxidant effects. ObjectiveThe objective of this study was to develop and optimise the synthesis of silver nanoparticles using flower extracts of Woodfordia fruticosa through the distillation extraction method. By employing this technique, the research aimed to successfully create silver nanoparticles from Woodfordia fruticosa gel for the treatment of wounds. MethodsWF flower extracts were prepared by drying the flowers for 14 days and crushing them into a fine powder. The powdered flowers were then sieved through 80-inch sievesThe extracted material was stored at 4 ​°C for further processing. In parallel, a 1 ​mM silver nitrate solution was prepared by dissolving 16.99 ​mg of silver nitrate in 100 ​ml of distilled water. The flower extract and silver nitrate solution were then utilised for the synthesis of silver nanoparticles. The characterization of the synthesised silver nanoparticles was conducted using various techniques, including zeta potential analysis and electron microscopy. These techniques were employed to assess the physicochemical properties, stability, and morphology of the silver nanoparticles. After gel preparation, in vivo wound healing activity was performed. ResultThe synthesis of AgNPs using WF flower extracts and silver nitrate solution resulted in the successful formation of nanoparticles. The characterization of the synthesised AgNPs was carried out using various techniques. The zeta potential was found to be −0.283 ​mV, which indicated their stability, and the PDI value was found to be 0.533. These values indicated a stable dispersion of the synthesised AgNPs. Scanning electron microscopy (SEM) showed that nanoparticles have a spherical shape in the range of 5 ​nm. ConclusionIn conclusion, this study successfully developed silver nanoparticles using flower extracts. The developed gel shows that it has wound-healing properties.