Abstract
The combination of ascorbic acid and rutin, often used in oral preparations, due to antioxidant and anti-inflammatory properties, can be used to protect skin cells against the effects of UV radiation from sunlight. Therefore, the aim of this study was to investigate the synergistic effect of rutin and ascorbic acid on the proteomic profile of UVA and UVB irradiated keratinocytes cultured in a three-dimensional (3D) system. Results showed that the combination of rutin and ascorbic acid protects skin cells against UV-induced changes. In particular, alterations were observed in the expression of proteins involved in the antioxidant response, DNA repairing, inflammation, apoptosis, and protein biosynthesis. The combination of rutin and ascorbic acid also showed a stronger cytoprotective effect than when using either compound alone. Significant differences were visible between rutin and ascorbic acid single treatments in the case of protein carboxymethylation/carboxyethylation. Ascorbic acid prevented UV or rutin-induced protein modifications. Therefore, the synergistic effect of rutin and ascorbic acid creates a potentially effective protective system against skin damages caused by UVA and UVB radiation.
Highlights
Maintaining the appropriate skin proteomic profile is a critical parameter for proper cell function and maintenance of healthy skin
The results of this study showed that rutin and ascorbic acid treatments lead to significant changes in protein expression in UVA or UVB irradiated keratinocytes
Comparing the p-values allowed us to identify which proteins had the highest differences in expression following rutin and ascorbic acid treatment (Figure 5)
Summary
Maintaining the appropriate skin proteomic profile is a critical parameter for proper cell function and maintenance of healthy skin For this reason, skin cells are characterized by the well-developed cytoprotective system, comprised of antioxidant, anti-inflammatory, and anti-apoptotic proteins. Skin cells are characterized by the well-developed cytoprotective system, comprised of antioxidant, anti-inflammatory, and anti-apoptotic proteins This protection system involves active cytoprotective transcription factors which are responsible for protein biosynthesis [1]. UV enhances ROS-dependent modifications in skin cells covering all cellular components including nucleic acids, proteins, and lipids. This is seen in lipid modification which causes an increase in the level of lipid peroxidation products, including 4-hydroxynonenal (4-HNE), which functions as an important signaling molecule [4]. Generation of CML leads to dysfunction of the proteins biological functions, their translocation into the cell
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