Abstract
Synergistic Ca2+ Responses by Gαi- and Gαq-coupled G-protein-coupled Receptors Require a Single PLCβ Isoform That Is Sensitive to Both Gβγ and Gαq
Highlights
Cross-talk between G␣i- and G␣q-linked G-protein-coupled receptors yields synergistic Ca2؉ responses in a variety of cell types
Because phospholipase C (PLC)4 appears to have an inhibitory role and PLC1 is not expressed in macrophages, the residual Ca2ϩ synergy in phospholipase C3 (PLC3)-deficient macrophages was thought to depend on PLC2 [11]
PLC2, like PLC3, has the capacity to mediate synergy in these cells. Both the G␥ and G␣q Binding Sites of PLC3 Are Required for Synergistic Ca2ϩ Responses—PLC3 is responsive to both G␥ and G␣q, and our studies suggest that synergy by G␣i and G␣q GPCRs may be mediated by the combined action of G␥ and G␣q on a single PLC isoform
Summary
Cross-talk between G␣i- and G␣q-linked G-protein-coupled receptors yields synergistic Ca2؉ responses in a variety of cell types. Macrophages Deficient in Both PLC2 and PLC3 Have No Synergistic Ca2ϩ Response to C5a and UDP—Synergy in Ca2ϩ signaling in macrophages between C5a, acting on the G␣icoupled C5aR, and UDP, acting on G␣q-coupled P2Y receptors, was previously found to be primarily dependent on expression of PLC3, but some synergy remained in PLC3-deficient cells [11].
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