Synergetic enhancement of methane production and system resilience during anaerobic digestion of food waste in ammonia-tolerant anaerobic sludge system.

Abstract

The anaerobic digestion (AD) of food waste (FW) was augmented with ammonia-tolerant anaerobic sludge (ATAS). Different inoculum substrate ratios (ISR) under an initial ammonia stress of 4220 mg N/L were investigated. Results showed that the average specific methane production (SMP) of FW in the ATAS system increased by 36% compared with that in un-acclimated anaerobic sludge. SMP with ISR of 1:2.5 increased by approximately 6 times. Volatile fatty acids (VFAs) accumulation and sharp pH decline were not detected. These results revealed the high performance of ATAS in simultaneously relieving ammonia and acid stress. This improvement was attributed to multiple factors. ATAS had high ammonia tolerance and ability in conversion of acetate into methane. The equilibrium of NH3/NH4+, CO2/H2CO3/HCO3-, and CxHyCOOH/CxHyCOO- could promote VFAs and ammonia ionization, reduce the levels of free VFAs and ammonia, neutralize pH, and thus enhance the system's buffering capacity to be less susceptible to fluctuations. These results demonstrated that employing ATAS in improving AD performance and resilience from acid and ammonia inhibition is feasible and effective.