Synchronous control of magnetic particles and magnetized cells in a tri-axial magnetic field.

Abstract

Precise manipulation of single particles is one of the main goals in the lab-on-a-chip field. Here, we present a microfluidic platform with "T" and "I" shaped magnetic tracks on the substrate to transport magnetic particles and magnetized cells in a tri-axial time-varying magnetic field. The driving magnetic field is composed of a vertical field bias and an in-plane rotating field component, with the advantage of lowering the attraction tendency and cluster formation between the particles compared to the traditional magnetophoretic circuits. We demonstrate three fundamental achievements. First, all the particle movements are synced with the external rotating field to achieve precise control over individual particles. Second, single-particle and single living cell transport in a controlled fashion is achieved for a large number of them in parallel, without the need for a complicated control system to send signals to individual particles. We carefully study the proposed design and introduce proper operating parameters. Finally, in addition to moving the particles along straight tracks, transporting them using a ∼60° bend is demonstrated. The proposed chip has direct applications in the fields of lab-on-a-chip, single-cell biology, and drug screening, where precise control over single particles is needed.