Abstract
The synchronization of somatic embryo development in embryogenic suspension cultures is a crucial step in taking advantage of somatic embryogenesis for high production potential and reduction of unit cost through automation. In the present study, a synchronous somatic embryogenic system was developed for Fraxinus angustifolia suspension cultures. High cell density, 6-benzyladenine (BA) and 2,4-dichlorophenoxyacetic acid proved essential for the establishment and maintenance of suspension cultures. Low cell density, BA and 1-naphthaleneacetic acid enhanced somatic embryo development. Cell and cell cluster fractionation by density gradient centrifugation in Ficoll solution proved useful for separation of subpopulations with differing potentials for embryo development. A synchronous development of somatic embryos at high frequency was achieved only from the heaviest cell population.
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