Two-Dimensional Protein Pattern Analysis of Extracellular Proteins Secreted by Embryogenic and Non-Embryogenic Suspension Cultures of Dactylis Glomerata L.

Abstract

ABSTRACTProteins from the medium of orchardgrass (Dactylis glomerata L.) embryogenic suspension culture during defined stages of somatic embryogenesis were compared with those of non-embryogenic suspension culture during unorganized cell proliferation. After separation on two-dimensional polyacrylamide gel electrophoresis, we were able to classify 40 proteins in each of 6 groups: a group common to both embryogenic and non-embryogenic suspension cultures, a group specific only for embryogenic, respectively non-embryogenic suspension cultures and 3 groups of microcluster-, proembryogenic masses- and somatic embryo-specific proteins. One of the groups is particularly interesting as it corresponds to polypeptides that are related to the earliest stages of somatic embryo-genesis-the transition of microclusters into proembryogenic masses when no morphological changes are visible yet. Using this experimental approach, we identified the major extracellular proteins involved in somatic embryo development and we discuss on the possibility for their use as early markers for somatic embryogenesis.