Abstract
Synaptotagmin 1 (Syt1) is a major Ca2+-sensor that evokes neurotransmitter release. Here we used site-specific fluorescence resonance energy transfer (FRET) assay to investigate the effects of Syt1 on SNAREpin assembly. C2AB, a soluble version of Syt1, had virtually no stimulatory effect on the rate of the FRET at N-terminus of SNARE complex both with and without Ca2+, indicating C2AB does not interfere with the initial nucleation of SNARE assembly. However, C2AB-Ca2+ accelerated the FRET rate significantly at membrane proximal region, indicating C2AB-Ca2+ promotes the transition from a partially assembled SNARE complex to the fusion-competent SNAREpin. Similar enhancement was also observed at the end of the transmembrane domain of SNARE proteins. The stimulatory effect disappeared if there was no membrane or only neutral membrane present.
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