Synaptic Vesicle Protein 2 (SV2) Isoforms

Cindy Bandala,S Juarez-Mendez,M Anaya-Ruiz,A Miliar-Garcia,M Gomez-Lopez,C.M Mejia-Barradas,E Lara-Padilla,C.I Bazan-Mendez,J.P Luna-Arias

doi:10.7314/apjcp.2012.13.10.5063

Abstract

New molecular markers of cancer had emerged with novel applications in cancer prevention and therapeutics, including for breast cancer of unknown causes, which has a high impact on the health of women worldwide. The purpose of this research was to determine protein and mRNA expression of synaptic vesicle 2 (SV2) isoforms A, B and C in breast cancer cell lines. Cultured cell lines MDA-MB-231, SKBR3, T47D were lysed and their protein and mRNA expression analyzed by real-time PCR and western blot technique, respectively. SV2A, B proteins were identified in non-tumor (MCF-10A) and tumor cell lines (MDA-MB-231 and T47D) while SV2C only was found in the T47D cell line. Furthermore, the genomic expression was consistent with protein expression for a such cell line, but in MDA-MB-231 there was no SV2B genomic expression, and the SV2C mRNA and protein were not found in the non tumoral cell line. These findings suggest a possible cellular transdifferentiation to neural character in breast cancer, of possible relevance to cancer development, and point to possible use of SV2 as molecular marker and a vehicle for cancer treatment with botulinum toxin.

Highlights

Breast cancer (BCa) in women is the most prevalent oncologic disease worldwide
SV2A, B proteins were identified in non-tumor (MCF-10A) and tumor cell lines (MDA-MB-231 and T47D) while SV2C only was found in the T47D cell line
The genomic expression was consistent with protein expression for a such cell line, but in MDA-MB-231 there was no SV2B genomic expression, and the SV2C mRNA and protein were not found in the non tumoral cell line

Summary

Introduction

Breast cancer (BCa) in women is the most prevalent oncologic disease worldwide. In Mexico, BCa presents an increase in mortality ratio without plenty knows of cause (McPherson et al, 2000). The presence of secretory vesicles is characteristic in synaptic (de Groot et al, 2010; 2011), gastric (Bumming et al, 2007), pancreatic (Jakobsen et al, 2002), adrenal (Li et al, 1999) and prostatic (Karsenty et al, 2009) cells which synaptic vesicular receptor (SV) play an important role in exocytosis and secretory process of synaptic (de Groot et al, 2010) and endocrine cells, (Dong et al, 2006; Coelho et al, 2010), but when cancer occurs this protein tends to overexpress in brain (de Groot et al, 2010), neuroendocrine (Portela-Gomes et al, 2000), gastrointestinal (Bumming et al, 2007) and prostate (Karsenty et al, 2009) tumors. BoNTA inhibits the growth of LNCaP human prostate cancer cells in vitro and in vivo (Karsenty et al, 2009)

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