Abstract
The present study is concerned with the immobilization of β-amylase from sweet potato tuberous roots on chitosan beads for the purpose of maltose production. Immobilized sweet potato β-amylase on Chitopearl BCW 3505 beads exhibited an activity of 142 U/g carrier. The optimum temperature of the immobilized β-amylase increased by 20°C, and thermostability was improved by about 10°C compared to free β-amylase. The additional effects of two types of the immobilized pullulanases on maltose formation were also investigated. The addition of pullulanase from Bacillus brevis gave a higher yield of maltose compared to that from Klebsiella pneumoniae. A semi-continuous production of maltose from a highly concentrated substrate (Pine-Dex #1, potato starch hydrolyzate) solution (40%, w/v) with the immobilized sweet potato β-amylase and Bacillus brevis pullulanase could be obtained at pH 6.0 and 60°C for 14 days at a higher yield (70.9%) using a horizontal rotary column reactor.
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
