Sweet Corn Stalk Treated with Saccharomyces Cerevisiae Alone or in Combination with Lactobacillus Plantarum: Nutritional Composition, Fermentation Traits and Aerobic Stability.

Zhou Zhou,Teklebrhan Teklebrhan,Tan Tan,Zhang Zhang,Ouyang Ouyang,Wei Wei,Zhu Zhu,Tang Tang,Ma Ma,Han Han

doi:10.3390/ani9090598

Abstract

Simple SummaryThe inclusion of Saccharomyces in the ration is beneficial to ruminants. We investigated the effects of inoculating a high-dose S. cerevisiae (108 cfu/g) on the nutritional composition and fermentation traits of sweet corn stalk. A high-dose S. cerevisiae inoculum increased the crude protein concentration of sweet corn stalk silage but decreased the silage quality. Thus, a high-dose S. cerevisiae inoculum is not conducive to obtaining high-quality corn stalk silage.This study examined the effects of a high-dose Saccharomyces cerevisiae inoculant alone or jointly with Lactobacillus plantarum on nutrient preservation, fermentation quality, and aerobic stability of sweet corn stalk silage. Fresh stalks (231 g dry matter (DM)/kg) were chopped and subjected to the following treatments: (1) deionized water (Uninoculated; U); (2) S. cerevisiae at 1 × 108 cfu/g of fresh forage (S); and (3) S. cerevisiae at 1 × 108 cfu/g plus L. plantarum at 1 × 105 cfu/g (SL). Treated stalks were ensiled in 5-litre laboratory silos for 30, 60, and 90 day. The S and SL silages had a greater (p < 0.001) pH and greater crude protein, ammonia nitrogen/total nitrogen, neutral detergent fibre, acid detergent fibre, and ethanol contents at all three ensiling periods than the U silage. Acetate, propionate and volatile fatty acids in the S and SL silages after 30 and 90 day of ensiling were greater (p < 0.05) than those in the U silage, but they were lower (p < 0.05) in the S and SL silages than in the U silage after 60 day. The lactate and V-score of the S and SL silages were lower (p < 0.001) than those of the U silage at all three ensiling periods. Compared with the U group, the aerobic stability of the S silage after 90 day of ensiling decreased (p < 0.05), and the aerobic stability of the SL silage was unaffected (p > 0.05). Overall, the quality of sweet corn stalk silage was not improved by inoculation with 108 cfu/g of S. cerevisiae alone or in combination with 1 × 105 cfu/g of L. plantarum.

Highlights

The fresh sweet corn stalk, after harvesting the cob, is rich in protein, starch, and water-soluble carbohydrates [1], which are used as unconventional fodder in herbivores [2]
The use of S. cerevisiae as a silage inoculant that can maintain or even enhance the number of viable cells is of great interest, as this will facilitate feeding management of S. cerevisiae and increase the feeding value of silage
The present study indicated that the S. cerevisiae inoculant at a rate of 108 cfu/g alone or together with L. plantarum affected the pH, chemical composition, organic acid profile, and ethanol content of corn stalk silage and led to a lower V-score

Summary

Introduction

The fresh sweet corn stalk, after harvesting the cob, is rich in protein, starch, and water-soluble carbohydrates [1], which are used as unconventional fodder in herbivores [2]. The use of the first three generations of [6] inoculants The current demand for the generation of silage inoculants is to further enhance the nutritional quality and to regulate intestinal microflora. Saccharomyces cerevisiae is a candidate microorganism with rich vitamins and growth factors [7]. It aids in modulating the immune system of young animals [8], improving rumen fermentation [9,10], and enhancing nutrient degradability of roughage in the hindgut [11]. The extracted DNA was diluted with ultrapure water to 1 ng/μL, and PCRs were performed in triplicate. The PCR products were excised from 2% agarose gels and purified using a QIAquick Gel extraction kit (QIAGEN GmbH)

Methods

Results

Conclusion