Sustained Protraction Increases Lengthening of the Mandibular Condylar Process Whilst Changes Its Growth Direction in a Rat Model.

Abstract

In Class II elastics treatment, the mandible is subject to sustained protraction. The study aimed to investigate the morphological and histological changes resulting from sustained mandibular protraction, through establishment of a novel model in rat. Forty-eight 4-week-old male Sprague-Dawley rats were randomly divided into three groups, including the 50 and 100 cN protraction groups, and the control. In the two protraction groups, NiTi coil springs were fixed between the metal bands on the maxillary incisors and mandibular molars to pull the mandible forward. CBCT scans were taken immediately before (T0), 2 weeks (T1) and 4 weeks (T2) after the traction. Histological and immunohistochemical analyses were performed at T1 and T2. Increase of the condylar process length and angular process length were significantly larger in the two protraction groups than that in the control; however, the condylar process angle (∠CPA) was significantly reduced by the protraction at T2, leading to decreased condylar height. The protraction decreased the condylar cartilage thickness at T1, which recovered to the control level at T2, with no significant changes detected in the expression of SRY-related high mobility group-box gene 9 (SOX9) and type II collagen (COL II), two chondrogenic markers. The established rat model is manoeuvrable and reliable, which exerts sustained and measurable mandibular protraction. The sustained protraction increases the lengthening of the mandibular condylar process, but alters its growth direction by causing it to turn downwards.