Abstract
The current study was performed to test the ability of the Pediococcus pentosaceus NCDC 273 strain for rapid utilization of lactose from paneer whey. We optimized the lactose utilization by monitoring the constitutive expression of the β-gal gene using real-time RT-PCR and also quantified lactic acid production using high-performance liquid chromatography (HPLC). Screening of β-gal gene for lactose utilization efficiency in P. pentosaceus NCDC 273 strain efficiency showed a significant (P < 0.0001) expression of the β-gal gene in the supplemented paneer whey medium (SPWM; lactose rich) compared to commercially available Deman-Rogosa-Sharpe (MRS; d-glucose rich) broth medium. In addition, with the growth of P. pentosaceus NCDC 273 in SPWM, the expression of the β-gal gene was found significantly (P < 0.001) higher at 6h of fermentation during the exponential growth phase and declining no significantly at stationary phase. After 48 h of incubation, HPLC data revealed a high lactose conversion rate (42.12%) to lactic acid production (14.50 ± 0.48 g L−1). Moreover, pediocin PA-1 activity in fermented SPWM showed almost contamination-free lactic acid production. Hence, our results showed the sustainable utilization of dairy waste paneer whey for lactic acid production by P. pentosaceus NCDC 273 at a lower disposal cost.
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