Sustainable green synthesis of silver nanoparticles using Chloris Barbata leaf extract with its bioactivity against multidrug resistant bacterial strains and as an antioxidant agent

Abstract

The microbial resistance conflicts have prompted the search for alternative medicine to resolve the alarming effect of antibiotic resistance. The method of preparation of nanoparticles using biological resources has been gaining a lot of attention with a wide spectrum of uses. In this work currently, we reported the study of Silver Nanoparticles (AgNPs) using the leaf extract of Chloris Barbata. The synthesized AgNPs have been examined using UV –Visible spectroscopic analysis, TEM, XRD, and FTIR. The development of the peak at 420 nm in the UV–Vis spectra for the formed AgNPs indicates the reduction of Ag+ ions into metal NPs (AgNPs). The crystalline nature of AgNPs was investigated by studying XRD analysis. The TEM images show that the AgNPs prepared were within 9 ± 2 nm size. In the determination of the potential functional groups, of the extract which brings out the process of reduction in the synthesis of AgNPs, the analysis of FTIR spectra was performed. The stability of AgNPs, investigated by Zeta potential were found to be –24 mV which confirmed their high stability. Furthermore, both gram-positive and gram-negative bacteria were used to test the antibacterial efficacy of the green-synthesized AgNPs. The synthesized AgNPs showed highly effective antioxidant activity. In the development of antimicrobial and antioxidant agents, the synthesized nanoparticles were a promising alternative.