Abstract
Phytobased bioactive compounds are herbal scavengers and beneficial in skin disorders, aging and oxidative damage. Himalayan Cherry (Prinsepia utilis) is a good source of fatty acids; however, screening and optimization of polyphenolics in leftover waste of seeds after oil extraction have not been studied. The present study focuses on the optimization of antioxidant polyphenolics of leftover oil residue of Prinsepia utilis. The leftover oil residue optimized through Response Surface Model (RSM) using ultrasonic assisted extraction (UAE) with four-factors in Plackett-Burman (PBD) and three-factors in Central composite design (CCD). The model showed good fitness under optimum extraction conditions and total phenolics, flavonoids, tannins, and antioxidant activity were closely fit with the model predicted values. HPLC-PDA analysis detects ten polyphenolic compounds under optimized extraction conditions. The anti-aging activity by tyrosinase and hyaluronidase indicated that extract is good in inhibiting tyrosinase (IC50-1.81 ± 0.02 mg/mL) and hyaluronidase (IC50-0.90 ± 0.03 mg/mL) enzyme. The extract prevented UV damage and showed good sun protection (SP) at value of 11.00 ± 0.03 at a dose of 1000 μg/mL. The study provides the potential use of leftover residue in water for obtaining antioxidant activity and alleviating oxidative stress, which will be beneficial in the prospect of phyto-nutrients at the Industrial level. Also, the method of extraction was used to promote green extraction, which can be sustainably used for harnessing the waste residue in developing byproducts such as nutraceuticals and cosmeceuticals in a safer environment.
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