Susceptibility of Fungi Infect Animals to Antimycotics in Alexandria City

Abstract

The aim of the present study was the isolation and phenotypic identification of dermatophytes isolated from cats, dogs, and cattle, followed by an evaluation of their antifungal susceptibility to azole antifungal agents in addition to isolation and phenotypic identification of Candida albicans from canine otitis, Aspergillus fumigatus from feline otitis and evaluation of their antifungal susceptibility. Specimens from pet animals were collected from veterinary clinics and from rural areas for the specimens from cattle in Alexandria city, Egypt. One hundred and ninety-five clinical samples were collected from pet animals and cattle (from cats; 80 ear swabs, 20 hair and skin scraping, from dogs; 50 ear swabs,15 hair and skin scraping and from cattle 30 hair and skin scraping) for mycological examination and antimycotic sensitivity test by disk diffusion method. The results of this study revealed that most dermatophytes isolated from pet animals are Microsporum canis, Microsporum gypsum, Trichophyton mentagrophytes, and Trichophyton rubrum, while from cattle M.gypseum, T.mentagrophytes and T.rubrum. Antifungal sensitivity test on the isolated dermatophytes was nearly similar and showed high resistance to fluconazole. In canine otitis, C.albicans was resistance to fluconazole, ketoconazole and clotrimazole and susceptible to itraconazole and voriconazole. A.fumigatus was susceptible only to itraconazole ,voriconazole and clotrimazole and resistance to other azole agents . This study shows the importance of knowing the main causative agent in canine and feline otitis and dermatophytosis in domestic animals and performing antifungal sensitivity to help in assaying the most susceptible antifungal agents.