Abstract
Dark chlorophyll accumulation in pine (Pinus pinea and Pinus nigra) and barley (Hordeum vulgare) seedlings was inhibited by gabaculine (3-amino 2,3-dihydrobenzoic acid) (GAB). The inhibitory effect of GAB was overcome by the addition of 5-aminolevulinic acid (ALA). Since GAB specifically interferes with ALA synthesis via the C5 (but not Shemin) pathway we conclude that ALA for light-independent chlorophyll synthesis in angiosperms and gymnosperms is formed via this route.
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