Abstract
Dark chlorophyll accumulation in the first leaf of intact 5–6-day-old glasshouse-grown barley seedlings ( Hordeum vulgare) was inhibited by gabaculine (3-amino 2,3-dihydrobenzoic acid) (GAB) supplied via the roots. The inhibitory effect of gabaculine was eliminated by the addition of 5-aminolevulinic acid. Gabaculine also inhibited chlorophyll accumulation in intact dark-grown barley seedlings transferred to light. Inhibition of dark chlorophyll accumulation by gabaculine is evidence that the aminolevulinic acid precursor for light-independent chlorophyll synthesis is formed via the C-5 pathway.
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