Abstract

Objective To investigate the effect of Survivin in the occurrence and development of endometrial carcinoma and its relationship with ERK/E-cadherin signaling pathway. Methods Immunohistochemistry was used to examine the expression of Survivin in endometrial carcinoma and adjacent tissues. Recombinant plasmid pRNAT-suv containing Survivin-specific siRNA was transfected into endometrial carcinoma cell line KLE. The mRNA expression level of Survivin was determined by RT-PCR before and after the transfection. CCK-8 method was used to examine the cell proliferation after Survivin interference on the expression. Transwell method was used to examine the cell migration after Survivin interference on the expression. Western blotting assay was used to examine the differences in the protein expression of β-catenin, cyclin D1, c-myc, p-ERK, Slug and E-cadherin between the groups before and after transfection. Results The expression level of Survivin in the endometrial carcinoma tissues was significantly higher than that in the adjacent tissues (P<0.05) . The expression level of Survivin in poorly differentiated endometrial carcinoma was higher than that in the well and moderately differentiated endometrial carcinoma (P=0.03) . The expression level of Survivin in deep myometrial invasive carcinoma was higher than that in superficial myometrial invasive carcinoma (P=0.04) . After Survivin interference on the expression, the cell proliferation and migration significantly decreased (P<0.01) . The findings of Western blotting results showed that after Survivin interference, the expression levels of β-catenin and its downstream proliferation-related molecules cyclin D1 and c-myc were down-regulated, the expression levels of p-ERK, Slug and metastasis-related proteins were significantly down-regulated, and the expression level of E-cadherin was up-regulated. Conclusion The high expression of Survivin is closely related to the occurrence and development of endometrial carcinoma. This signaling pathway study revealed that Survivin may promote the proliferation and metastasis of endometrial carcinoma cells mainly via ERK/E-cadherin pathway. Key words: Survivin; ERK/E-cadherin; Endometrial carcinoma; Proliferation; Migration

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