Abstract
A ring pump (RP) is a useful tool for microchannels and automated cell culturing. We have been developing RPs (a full-press ring pump, FRP; and a mid-press ring pump, MRP). However, damage to cells which were sent by the RP and the MRP was not investigated, and no other studies have compared the damage to cells between RPs and peristaltic pumps (PPs). Therefore, first, we evaluated the damage to cells that were sent by a small size FRP (s-FRP) and small size MRPs (s-MRPs; gap = 25 or 50 μm, respectively). “Small size” means that the s-FRP and the s-MRPs are suitable for microchannel-scale applications. The survival rate of cells sent by the s-MRPs was higher than those sent by the s-FRP, and less damage caused by the former. Second, we compared the survival rate of cells that were sent by a large size FRP (l-FRP), a large size MRP (l-MRP) (gap = 50 μm) and a PP. “Large size” means that the l-FRP and the l-MRP are suitable for automated cell culture system applications. We could not confirm any differences among the cell survival rates. On the other hand, when cells suspended in Dulbecco’s phosphate-buffered saline solution were circulated with the l-MRP (gap = 50 μm) and the PP, we confirmed a difference in cell survival rate, and less damage caused by the former.
Highlights
Various pumps have been considered for biotechnological utilizations
We considered reasons why the survival rate of cells that were sent by the PP was lower than that of the large size mid-press RP (MRP) (l-MRP) from the viewpoint of fluid dynamics
We evaluated the damage to cells which were sent with the size full-press RP (FRP) (s-FRP) and the size MRPs (s-MRPs)
Summary
Various pumps have been considered for biotechnological utilizations. A centrifugal pump, a peristaltic pump (PP), a diaphragm pump, a monoscrew pump, a rotary bane pump and an eccentric rotor pump were evaluated for the cell concentration process in [1]. A centrifugal pump, a PP, a gravity-driven pump, a surface tension-based pump, an osmosis-based pump, a syringe pump, a vacuum pump and an electrokinetic pump were employed for microfluidic cell culturing using microfluidic devices [2]. Microfluidic platforms (microfluidic devices) have advantages of small size and high throughput experimentation [2]. Because microfluidic devices can control chemical and physical environments, these devices have been applied to small-scale cell cultivation systems such as Micromachines 2020, 11, 447; doi:10.3390/mi11040447 www.mdpi.com/journal/micromachines. Micromachines 2020, 11, 447 Micromachines 2020, 11, x. Bkyinsgtrtohkeiinr gtutbheesirwtuitbhersowlleitrhs,rPoPllsercsa,nPsPesncdansosleuntidonsoslwutiitohnosuwt cihthaonugtincghathnegisnogutrhcee osof ulirqcueidofalniqdutihdeaPnPdstchaenPsPensdcatnhesesnoldutthioensionluatgiaoinn.inBaagseadino. nBathseedseopnrothpeesretiepsr,oPpPesrthieasv,ePbPesehnauvseedbefeonr cuusletdivfaotriocnulstyivstaetmiosn[s1y5s–t1e7m] san[1d5–b1io7r]eaancdtobrsio[r9e,1a8c,t1o9r]s. [9,18,19]
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