Abstract
The aim of this work was to isolate endophytic bacteria from wheat grains and to evaluate their plant growth promoting traits (PGPT) as well as an inhibitory effect on P. syringae pv. atrofaciens (McCulloch) growth. Endophytic bacteria were isolated by a culture-dependent protocol from the grains of winter wheat variety of Ukrainian selection Podolyanka with high resistance to syringae. Totally 2.7±0.09 CFU/1 g of dry wheat grain were isolated, ten cultivable bacterial isolates were obtained. Spore-forming bacilli predominated in the wheat grain endophytic community. Gram-negative fermenting and non-fermenting rod-shaped bacteria and Gram-positive cocci were also present. Seven out of ten isolates possessed numerous plant growth promoting traits including phosphate solubilization, oligonitrotrophy, and indolic compound producing. Two isolates possessed antagoniscic activity against syringae in vitro along with plant growth promoting features. According to biochemical profiling and mass-spectrophotometric identification, these two isolates were assigned to Paenibacillus and Brevibacillus genera. These endophytic bacteria can be considered as promising objects for agrobiotechnology. However, more research is needed to confirm their biotechnological potential in planta experiments
Highlights
It is generally accepted, that bacterial endophytic communities are present in all plants [1, 2].The growing number of studies on endophytic bacteria has revealed their significance in agricultural production, food safety, and phytoremediation
Morphological, physiological and biochemical characters, and identification of cultivable endophytic bacteria from wheat grain Wheat grains were first rinsed with running tap water for 15 min, were surface asepticized with 72 % ethanol for 30 s, followed by treatment with 2 % trichloroisocyanuric acid for 4,5 min and – with 72 % ethanol for 30 s
In previous experiments we have revealed winter wheat variety of Ukrainian selection Podolianka with high resistance to P. syringae pv. atrofaciens (McCulloch) [22]
Summary
That bacterial endophytic communities are present in all plants [1, 2].The growing number of studies on endophytic bacteria has revealed their significance in agricultural production, food safety, and phytoremediation. That bacterial endophytic communities are present in all plants [1, 2]. Plant endophytic bacteria protect host from infectious diseases and herbivores, and even deter growth of competitor plant species [3, 4]. Endophytic bacterial communities of some root vegetables contain lactic acid bacteria, offering its use as a source of probiotic microorganisms [5]. Some endophytic bacteria exhibit organic pollutant degradation activity along with plant growth-promoting properties, and can be successfully applied for intensified phytoremediation [7]. All these features of endophytic bacteria position them as a promising object for biotechnology
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
