Survey and Diversity of Grapevine Pinot gris virus in Algeria and Comprehensive High-Throughput Small RNA Sequencing Analysis of Two Isolates from Vitis vinifera cv. Sabel Revealing High Viral Diversity.

Aleš Eichmeier,Jana Čechová,Akila Berraf-Tebbal,Eliška Peňázová

doi:10.3390/genes11091110

Aleš Eichmeier, Jana Čechová + Show 2 more

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https://doi.org/10.3390/genes11091110

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Journal: Genes	Publication Date: Sep 22, 2020
Citations: 5	License type: CC BY 4.0

Affiliation: Mendel University in Brno

Abstract

Grapevine Pinot gris virus (GPGV) is a putative causal agent of grapevine leaf mottling and deformation disease that has been reported worldwide throughout the grapevine-growing regions. Fifty-four grapevines collected from five Algerian grapevine-growing regions were tested for the presence of GPGV in phloem tissues. Eight of the tested grapevines were infected by GPGV. Viromes of two selected Vitis vinifera cv. Sabel grapevines infected by GPGV and showing virus-like symptoms were analyzed by small RNA sequencing. Phylogenetic analyses of the partial coding sequence (cds) of the RNA-dependent RNA polymerase (RdRp) domain showed that all Algerian GPGV isolates were grouped with some already-described asymptomatic isolates. This study provides the first survey of the occurrence of GPGV in Algeria. Moreover, Grapevine fleck virus, Grapevine rupestris stem pitting-associated virus, Grapevine virus B, Grapevine rupestris vein feathering virus, Hop stunt viroid and Grapevine yellow speckle viroid 1 were detected in Algeria for the first time.

Highlights

IntroductionGrapevine Pinot gris virus (GPGV) is a member of the genus Trichovirus in the family Betaflexiviridae
Grapevine Pinot gris virus (GPGV) is a member of the genus Trichovirus in the family Betaflexiviridae.The virus was initially described in the Italian grapevine cv
We found that the highest similarity of the complete cds RNA-dependent RNA polymerase (RdRp) domain of AL_41 was detected in an Italian GPGV isolate, fvg-Is14, which caused symptoms of grapevine leaf mottling and deformation disease (GLMD) [45]

Summary

Introduction

Grapevine Pinot gris virus (GPGV) is a member of the genus Trichovirus in the family Betaflexiviridae. The virus was initially described in the Italian grapevine cv. Pinot gris in which it causes grapevine leaf mottling and deformation disease (GLMD) [1,2]. GPGV is widespread in many wine-producing countries around the world [3], and was more recently detected in Canada [4], Armenia [5], Australia [6], Brazil [7], Chile [8] and Pakistan [9]. Several studies in Italy have monitored the production and growth parameters of GLMD-affected/GPGV-infected vineyards planted with varieties that are expected to be more sensitive to GPGV infection [2,11,12].

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