Surgical Modification of the Murine Calvaria Osteolysis Model.

Ali Mohammed Al-Quhali,Xizhuang Bai,Yu Sun,Zhe Jin,Guibo Yu

doi:10.1155/2015/802697

Ali Mohammed Al-Quhali, Xizhuang Bai + Show 3 more

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https://doi.org/10.1155/2015/802697

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Abstract

The murine calvaria model has been adopted for evaluation of osteolysis and inflammation induced by polyethylene (PE) or metal wear debris. However, this model suffers from several complications. The purpose of our study is to introduce a surgical modification with lower complication rates, thus providing more accurate results. Forty C57/BL6 mice were divided into two groups, both receiving polyethylene particles. Surgical modifications were performed in group 1, and group 2 underwent traditional surgeries. The incidence of fluid leakage was recorded on the operative day. Curst formation, wound dehiscence, and bone exposure were recorded on day 7. Histological osteolysis was demonstrated by HE staining of tissue slices. Micro-CT was used for quantifying evaluation of osteolysis in two groups. Intraoperative fluid leakage was significantly reduced in group 1. Postoperative crust formation, wound dehiscence, and bone exposure were also significantly decreased in group 1. HE staining results revealed obvious osteolysis in group 1 and more obvious osteolysis in group 2. Bone volume fraction (BVF) was (0.32 ± 0.03) in group 1 compared to group 2 (0.24 ± 0.05). Bone mineral density (BMD) was (1.11 ± 0.03) in group 1 compared to group 2 (1.01 ± 0.02). Surgical modifications provide a reliable way for establishment of the murine calvaria osteolysis model.

Highlights

Aseptic loosening of joint prosthesis induced by polyethylene (PE) and metal wear debris is a disastrous postoperative complication, leading to implant failure and revision surgery [1,2,3,4,5]
Due to the limitation of the access to human joint tissues and implanted prosthesis in early stages [9], the murine calvaria model introduced by Merkel et al using polyethylene particles [10] and these early previous studies [11] used the traditional surgical techniques for assessment of polyethylene and titanium particles
The purpose of this study is to introduce a surgical modification of the conventional murine calvaria osteolysis model with lower complication rates

Summary

Introduction

Aseptic loosening of joint prosthesis induced by polyethylene (PE) and metal wear debris is a disastrous postoperative complication, leading to implant failure and revision surgery [1,2,3,4,5]. Due to the limitation of the access to human joint tissues and implanted prosthesis in early stages [9], the murine calvaria model introduced by Merkel et al using polyethylene particles [10] and these early previous studies [11] used the traditional surgical techniques for assessment of polyethylene and titanium particles. This murine calvaria model has been widely adopted for evaluation of osteolysis and inflammation and is thought to be sensitive, costeffective, and time saving. The modification to the original technique may be seen to be minor; we believe that these changes are of importance and could better be taken into consideration in future research

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