Abstract
This investigation assessed the interaction of surface water samples with DNA to quantitatively and qualitatively characterize their mutagenic and/or recombinagenic activity. Samples were obtained at three different sites along the Tocantins River (Tocantins State, Brazil). The area has withstood the impact mainly of rural activities, which release different chemical compounds in the environment. The Drosophila melanogaster Somatic Mutation and Recombination Test (SMART) was performed in standard (ST) and high bioactivation (HB) crosses. SMART is useful for the detection of mutational and recombinational events induced by genotoxins of direct and indirect action. Results demonstrated that samples collected in both seasons were able to induce increments on the mutant spot frequencies in the larvae of the HB cross. Genotoxicity was related to a massive recombinagenic activity. The positive responses ascribed to only the HB cross means that it is linked to pro-genotoxins requiring metabolic activation. The SMART wing test in Drosophila melanogaster was shown to be highly sensitive to detect genotoxic agents present in the aquatic environment impacted by agriculture.
Highlights
Characterized as complex mixtures of various chemical compounds, pesticides are widely used in agriculture to control pests and weeds and for combating plant diseases
Aiming to look into the genotoxic condition of surface waters exposed to the influence of agricultural activities, the present study investigated the mutagenic and/or recombinagenic potential of samples collected at three different sites along the Tocantins River between November 2012 and
The results obtained in the present study show that the surface waters of Tocantins River are genotoxic to somatic cells of D. melanogaster
Summary
Characterized as complex mixtures of various chemical compounds, pesticides are widely used in agriculture to control pests and weeds and for combating plant diseases. Public Health 2016, 13, 827; doi:10.3390/ijerph13080827 www.mdpi.com/journal/ijerph
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More From: International Journal of Environmental Research and Public Health
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