Abstract
Objective: This study aimed to evaluate the influences of surface roughness (SR) of denture base and liner materials on Candida albicans biofilm formation.Methods: Discs were fabricated using poly (methyl methacrylate) acrylic resin and poly(ethyl methacrylate) denture liner, according to manufacturers’ instructions. Both acrylic resin and relined discs were finished or finished + polished and the SR was measured. Discs were saliva-coated, and C. albicans biofilms were developed on such surfaces for 48 hours. Biofilms were evaluated for cell counts, metabolic activity, and structural characteristics. Data were analyzed by one-way ANOVA and Tukey tests using a significance level of 5%.Results: Finished acrylic resin and denture liner discs had higher SR compared to finished + polished discs (P<0.001). Finished + polished acrylic resin discs contained fewer cells compared to only finished discs (P<0.001). No metabolic activity differences were seen between biofilms formed on both groups (P>0.05). Bulky, thick and less rough biofilms were formed on only finished denture materials (P<0.05). Confocal images reveal increased presence of black spaces for biofilms developed on finished + polished discs. Conclusion: Reduced SR resulted in decreased C. albicans biofilm accumulation on both denture materials.
Highlights
Candida albicans has been reported to play a major role in Candida-associated denture stomatitis (CADS) pathogenesis, and has been found on both poly(methyl methacrylate) (PMMA) acrylic resin and poly(ethyl methacrylate) (PEMA) liner materials [1,2,3,4,5,6,7]
The physical characteristics of denture liners make them susceptible to sorption and dimensional changes, which can result in porous surfaces that are colonized by fungal cells [5]
It was observed that finished + polished acrylic resin discs contained fewer cells compared to only finished discs (P
Summary
Candida albicans has been reported to play a major role in Candida-associated denture stomatitis (CADS) pathogenesis, and has been found on both poly(methyl methacrylate) (PMMA) acrylic resin and poly(ethyl methacrylate) (PEMA) liner materials [1,2,3,4,5,6,7]. C. albicans must first adhere to the denture surface [8] and the substratum characteristics, such as the surface roughness (SR), could influence this process. Denture materials with rougher surfaces have been shown to exhibit higher yeast counts [7]. PMMA denture bases with a SR above 0.2 μm have been shown to be readily colonized by C. albicans [6,7]. The physical characteristics of denture liners make them susceptible to sorption and dimensional changes, which can result in porous surfaces that are colonized by fungal cells [5]. Several studies have examined the role of SR in C. albicans adherence [2,3,7,8,9,10,11,12], the influence of SR on mature biofilms has been poorly investigated
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