Surface rheology of sorbitan tristearate and β-lactoglobulin: Shear and dilatational behavior

Abstract

Proteins and surfactants behave very differently under shear and dilatational deformation. In this work, we compare specifically their surface properties by evaluating their rheological response. Oil-soluble surfactant, sorbitan tristearate (Span 65), and globular protein, β-lactoglobulin, were spread and adsorbed onto the surface, respectively. A 2D searle-type measuring geometry with a biconical bob was used for measuring the surface shear rheology, and a pendant drop film balance was used for measuring the dilatational rheology. Both equipments provided the viscoelastic properties (surface shear and dilatational complex moduli) of interfacial layers. Also, the linear and non-linear rheology of these systems was studied by increasing the amplitude of the oscillation. Linear rheology showed that dilatational deformation is mostly affected by the nature of the molecular structure at the interface, whereas shear deformation is affected by the strength of the surface film due to the intermolecular interactions. Furthermore, large-amplitude oscillatory shear rheology indicated that the non-linearity increases with the surface concentration, and is higher for insoluble Span 65 spread films than for soluble protein adsorbed layers. Dilatational and shear deformation provide complementary information about interfacial layers that can be optimized so as to fully characterize the surface depending of the type of film (spread or adsorbed) and the technique used (shear or dilatational rheology under linear or non-linear regimes). This information is useful to correlate the structure and the mechanical properties of interfacial systems.