Abstract
The development of highly sensitive detection of [Formula: see text]-agonists compound by using surface plasmon resonance (SPR) immunoassay functionalized alkanethiols monolayer on gold surface was reported here. For the construction of robust SPR sensor surface, the illegal compound (as antigen) was immobilized onto gold succinimide-terminated monolayer to perform amide-coupling reaction. Structural characteristic of monolayer is an important issue for further application of selt-assembled monolayer (SAM)-based molecular electronics. This study focuses on the relation between structure of alkanethiols SAM of different chain length and its sensitivity of the detection. The surface structure is characterized by electrochemical methods and STM. The target compound in this research is clenbuterol as farmers used illegally to increase their profit gain. For the detection of clenbuterol, indirect competitive inhibition method was used. In this work, the SPR senses the dielectric constant change at the interface from the binding of the unreacted antibody to antigen-immobilized on the sensor surface. By this method, clenbuterol can be detected within ppt level and only requires 5[Formula: see text]min per sample. Furthermore, shorter chain alkanethiol sensor surface produces higher sensitivity in the detection process.
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