Abstract
The development of smart nanoparticles that can exhibit the anti-cancer activity, introduces better efficacy and lower toxicity for treatment. The present study was aimed to evaluate the anti-cancer activity of surface functionalized CoO nanoparticles against Jurkat (T-cell lymphoma) and KB (oral carcinoma) cell lines. The nano-sized cobalt oxide nanoparticles (CoO) was prepared by thermal decomposition method followed by surface modification using phosphonomethyl iminodiacetic acid (PMIDA). The PMIDA-coated CoO nanoparticle was characterized by X-ray diffraction, dynamic light scattering, and transmission electron microscopy; and the conjugation was analyzed by Fourier transform infrared spectroscopy. The resultant nanoparticles with an average size less than 100 nm measured by dynamic light scattering and transmission electron microscopy. Cytotoxicity study, flow cytometric analysis and scanning electron micrographs have been revealed that PMIDA-coated nanoparticles significantly enhances the cellular uptake of the nanoparticle and thus facilitates apoptosis of cancer cell (Jurkat and KB). For the application of PMIDA-coated CoO nanoparticles in the medical field, doxorubicin, a potent anti-cancer drug, has been used in similar fashion in this experimental design and all these effects or patterns were observed.
Highlights
The rapid developments of various modified nanoparticles (NP) are attracting increased attention because of their potential in a wide range of biotechnological applications, especially in advance biomedical application (Liong et al 2008), drug delivery systems (Cheng et al 2006), and vaccine administration (Schreiber et al 2010)
The nano-sized cobalt oxide nanoparticles (CoO) was prepared by thermal decomposition method followed by surface modification using phosphonomethyl iminodiacetic acid
It was found that there was no significant difference in cell viability between the cells treated with phosphonomethyl iminodiacetic acid (PMIDA)-coated CoO nanoparticles and the cell treated without PMIDA-coated CoO nanoparticles
Summary
The rapid developments of various modified nanoparticles (NP) are attracting increased attention because of their potential in a wide range of biotechnological applications, especially in advance biomedical application (Liong et al 2008), drug delivery systems (Cheng et al 2006), and vaccine administration (Schreiber et al 2010). (Uhrich et al 1999; Panyam and Labhasetwar 2003; Marin et al 2005; Marinakos et al 2001) Among these applications, the field of drug delivery by nanoparticles with specific and rapid internalization into a target cell has immense promise (Maeda et al 2009; Faraji and Wipf 2009; Singh and Lillard 2009; Breunig et al 2008; BrannonPeppas and Blanchester 2004). In the present paper, we have focused our interest on the study of modified CoO NPs. Believing that the characterization of the physical– chemical properties of NP is relevant for the study of their biological activity, we analyzed CoO NP morphology by electron microscopy in order to define aggregation, size, and shape. This present study was aimed to develop a novel and economically viable nanoparticles which have anti-cancer activity to cancer cell in vitro
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