Abstract
A sensitive and stable cellulose paper platform modified with carboxymethyl cellulose (CMC) was investigated for use as a rapid and accurate immunoassay for tuberculosis. Absorbing CMC to the paper substrate enables it to act as a linker for immobilizing biomolecules in a hydrophilic environment to promote their functionality and stability. This CMC-modified cellulose material can also be crosslinked with 1-ethyl-3-(-3-dimethylaminopropyl) carbodiimide hydrochloride/N-Hydroxy-succinimide (EDC/NHS) to generate NHS ester groups on the paper surface that can be covalently conjugated with ligands or target analytes for highly specific binding. As a result, with this system we were able to achieve a detection limit of 0.03 ng/mL for tuberculin-purified protein derivative. In addition, multiple tests can be completed simultaneously in as little as 21 min. These results demonstrate how this surface-modified paper analytical device can provide sensitive and high-throughput on-site disease diagnosis for resource-limited settings.
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